Indian idli is a small, white, acidic, leavened, steam-cooked cake made by lactic fermentation of a thick batter made from polished rice and dehulled black gram dhal, a pulse (Phaseolus mungo). The cakes are soft, moist, and spongy and have a pleasant sour flavor. Dosa, a closely related product, is made from the same ingredients, both finely ground. The batter is generally thinner, and dosa is fried like a pancake.

Idli fermentation is a process by which leavened bread-like products can be made from cereals other than wheat or rye and without yeast. The initial step in the fermentation is to wash both rice and black gram dhal. They are then soaked for 5 to 10 hours and drained. The coarsely ground rice and black gram are then combined with water and 1 percent salt to make a thick batter. The batter is fermented in a warm place (30 to 32°C) overnight, during which time acidification and leavening occur. The batter is then placed in small cups and steamed or fried as a pancake. The proportions of rice to black gram vary from 4:1 to 1:4, depending on the relative cost on the market.

Idli and dosa are both products of natural lactic acid fermentation. L. mesenteroides and S. faecalis develop during soaking, then continue to multiply following grinding. Each eventually reaches more than 1 × 109 cells per gram, 11 to 13 hours after formation of the batter. These two species predominate until 23 hours following batter formation. Practically all batters would be steamed by then. If a batter is further incubated, the lactobacilli and streptococci decrease in numbers and P. cerevisiae develops. L. mesenteroides is the microorganism essential for leavening of the batter and, along with S. faecalis,is also responsible for acid production. Both functions are essential for producing a satisfactory idli.

In idli made with a 1:1 ratio of black gram to rice, batter volume increased about 47 percent 12 to 15 hours after incubation at 30°C. The pH fell to 4.5 and total cidity rose to 2.8 percent (as lactic acid). Using a 1:2 ratio of black gram to rice, batter volume increased 113 percent and acidity rose to 2.2 percent in 20 hours at 29°C. Reducing sugars (as glucose) showed a steady decrease from 3.3 milligrams per gram of dry ingredients to 0.8 milligrams per gram in 20 hours, reflecting their utilization for acid and gas production. Soluble solids increased,
whereas soluble nitrogen decreased. Flatulence-causing oligosaccharides, such as
stachyose and raffinose, are completely hydrolyzed.

A 60 percent increase in methionine has been reported during fermentation. The increase would be of considerable nutritional importance if true, but the results conflict with earlier findings. Thiamine and riboflavin increases during fermentation and phytate phosphorous decreases have also been reported.

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Pickling of cucumbers and other vegetables is widely practiced today.Although a variety of techniques are used, placing cucumbers in a 5 percent salt brine is a satisfactory method. The cucumbers absorb salt until there is an equilibrium between the salt in the cucumbers and the brine. Acidity reaches 0.6 to 1.0 (as lactic acid) with a pH of 3.4 to 3.6 in about 2 weeks, depending on the temperature.

In Malaysia the most common vegetables pickled are cucumbers, ginger,onion, leek, chili, bamboo shoots, and leafy tropical vegetables like mustard leaves. Young unripe fruits commonly pickled include mangoes, papaya, pineapple, and lime. In Egypt carrots, cucumbers, turnips, cauliflower, green and black olives, onions, and hot and sweet peppers are among the vegetables pickled. They are used as appetizers and served with practically every meal.

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Korean kimchi differs from sauerkraut in two respects: it has, optimally,much less acid and it is carbonated. Chinese cabbage and radish are the major substrates; garlic, green onion, ginger, leaf mustard, hot pepper, parsley, and carrot are minor ingredients.

Kimchi is available year-round, is served three times daily, and is a diet staple along with cooked rice and certain side dishes. It accounts for about an eighth of the total daily food intake of an adult. Its popularity is largely due to its carbonation derived from fermentation with natural microflora.

Salting of the cabbage can be done at 5 to 7 percent salinity for 12 hours or 15 percent salinity for 3 to 7 hours, followed by rinsing and draining. Optimum salt concentration during kimchi fermentation is approximately 3 percent. Lower temperatures (about 10°C) are preferred to temperatures above 20°C. Optimum acidity of kimchi is 0.4 to 0.8 percent lactic acid with a pH between 4.2 and 4.5; higher acidity makes it unacceptable. Organisms isolated from kimchi include L. mesenteroides, S. faecalis, Lb. brevis, Lb. plantarum, and P. cerevisiae.

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Lactic acid fermentation of cabbage and other vegetables is a common way of preserving fresh vegetables in the western world, China, and Korea (where kimchi is a staple in the diet). It is a simple way of preserving food: the raw vegetable is sliced or shredded, and approximately 2 percent salt is added. The salt extracts liquid from the vegetable, serving as a substrate for the growth of lactic acid bacteria. Anaerobic conditions should be maintained, insofar as
possible, to prevent the growth of microorganisms that might cause spoilage.

The sequence of organisms that develop in a typical sauerkraut fermentation is as follows: Leuconostoc mesenteroides initiates the growth in the shredded cabbage over a wide range of temperatures and salt concentrations. It produces carbon dioxide and lactic and acetic acids, which quickly lower the pH, thereby inhibiting development of undesirable microorganisms that might destroy crispness. The carbon dioxide produced replaces the air and facilitates the anaerobiosis required for the fermentation. The fermentation is completed in sequence by Lactobacillus brevis and Lb. plantarum. Lb. plantarum is responsible for the high acidity. If the fermentation temperature or salt concentration is high, Pecicoccus cerevisiae develops and contributes to acid production.

As would be expected, the rate of completion of the fermentation depends on the temperature and salt concentration. At 7.5°C fermentation is very slow: under these circumstances, L. mesenteroides grows slowly, attaining an acidity of 0.4 percent in about 10 days and an acidity of 0.8 to 0.9 percent in a month. Lactobacilli and pediococci cannot grow well at this temperature, and the fermentation may not be completed for 6 months. At 18°C a total acidity (as
lactic acid) of 1.7 to 2.3 percent will be reached, with an acetic to lactic acid ratio of 1:4, in about 20 days. At 32°C a similar activity will be reached in 8 to 10 days, with most of the acid being lactic acid produced by the homofermentative bacteria Lb. plantarum and P. cerevisiae.

Increasing the salt concentration to 3.5 percent results in 90 percent inhibition of growth and acid production for both L. mesenteroides and Lb. brevis. The ratio of nonvolatile to volatile acid produced has a marked effect on flavor, Lb. brevis producing a harsh, vinegar-like flavor and L. mesenteroides a mild, pleasantly aromatic flavor. The homofermenters Lb. plantarum and P. cerevisiae yield unacceptable products.

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Keith H. Steinkraus

Lactic acid bacteria perform an essential role in the preservation and production of wholesome foods. The lactic acid fermentations are generally inexpensive, and often little or no heat is required in their preparation, making them fuel efficient as well. Foods fermented with lactic acid play an important role in feeding the world's population on every continent.

Lactic acid bacteria perform this essential function in preserving and producing a wide range of foods: fermented fresh vegetables such as cabbage (sauerkraut, Korean kimchi); cucumbers (pickles); fermented cereal yogurt (Nigerian ogi, Kenyan uji); sourdough bread and bread-like products made without wheat or rye flours (Indian idli, Philippine puto); fermented milks
(yogurts and cheeses); fermented milk-wheat mixtures (Egyptian kishk, Greek trahanas); protein-rich vegetable protein meat substitutes (Indonesian tempe); amino acid/peptide meat-flavored sauces and pastes produced by fermentation of cereals and legumes (Japanese miso, Chinese soy sauce); fermented cereal-fish-shrimp mixtures (Philippine balao balao and burong dalag); and fermented meats (e.g., salami).

Lactic acid bacteria are generally fastidious on artificial media, but they grow readily in most food substrates and lower the pH rapidly to a point where competing organisms are no longer able to grow. Leuconostocs and lactic streptococci generally lower the pH to about 4.0 to 4.5, and some of the lactobacilli and pediococci to about pH 3.5, before inhibiting their own growth.

In addition to producing lactic acid, lactobacilli also have the ability to produce hydrogen peroxide through oxidation of reduced nicotinamide adenine dinucleotide (NADH) by flavin nucleotide, which reacts rapidly with gaseous oxygen. Flavoproteins, such as glucose oxidase, also generate hydrogen peroxide and produce an antibiotic effect on other organisms that might cause food spoilage; the lactobacilli themselves are relatively resistant to hydrogen peroxide.

Streptococcus lactis produces the polypeptide antibiotic nisin, active against gram-positive organisms, including S. cremoris, which in turn produces the antibiotic diplococcin, active against gram-positive organisms such as S. lactis. Thus, these two organisms compete in the fermentation of milk products while inhibiting growth of other gram-positive bacteria.

Carbon dioxide produced by heterofermentative lactobacilli also has a preservative effect in foods, resulting, among others, from its flushing action and leading to anaerobiosis if the substrate is properly protected.

Brining and lactic acid fermentation continue to be highly desirable methods of processing and preserving vegetables because they are of low cost, have low energy requirements for both processing and preparing foods for consumption, and yield highly acceptable and diversified flavors. Depending on the salt concentration, salting directs the subsequent course of the fermentation, limiting the amount of pectinolytic and proteolytic hydrolysis that occurs, thereby
controlling softening and preventing putrefaction. Lactic acid fermentations have other distinct advantages in that the foods become resistant to microbial spoilage and toxin development. Acid fermentations also modify the flavor of the original ingredients and often improve nutritive value.

Because canned or frozen foods are mostly unavailable or too expensive for hundreds of millions of the world's economically deprived and hungry people, acid fermentation combined with salting remains one of the most practical methods of preservation, often enhancing the organoleptic and nutritional qualities of fresh vegetables, cereal gruels, and milk-cereal mixtures.

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