Book Review:

Directed Enzyme Evolution: Screening and Selection Methods
(Methods in Molecular Biology)
From Humana Press


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Product Description
Seasoned practitioners from many leading laboratories describe their best readily reproducible screening strategies for isolating useful clones. These techniques have been optimized for sensitivity, high throughput, and robustness, and are of proven utility for directed evolution purposes. The assays presented use a variety of techniques, including genetic complementation, microtiter plates, solid-phase screens with colorimetric substrates, and flow cytometric screens. An accompanying volume, Directed Evolution Library Creation: Methods and Protocols (ISBN 1-58829-285-1), describes readily reproducible methods for the creation of mutated DNA molecules and DNA libraries.

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Directed Evolution Library Creation: Methods and Protocols and Directed Enzyme Evolution: Screening and Selection Methods constitute an extraordinary collection of all the key methods used today for directed evolution research. Described in step-by-step detail to ensure robust experimental results, these methods will enable both newcomers and more experienced investigators to design and implement directed evolution strategies for the engineering of novel proteins. The first volume describes methods for the creation of mutated DNA molecules, or DNA libraries, encoding variants of desired proteins. The second volume describes methods for screening DNA libraries to isolate mutant proteins that exhibit a specified function.

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Product Details
Amazon Sales Rank: #869739 in Books
Published on: 2003-05-16
Original language: English
Number of items: 1
Binding: Hardcover
370 pages

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Editorial Reviews
Review
"...covers a considerable number of protocols for a broad range of enzymes...very useful..." - ChemBioChem

covers a considerable number of protocols for a broad range of enzymes very useful ChemBioChem

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