Enzyme Assays: A Practical Approach
(The Practical Approach Series, 257)
From Oxford University Press, USA
Product Description
Enzyme assays are among the most frequently performed procedures in biochemistry and are routinely used to estimate the amount of enzyme present in a cell or tissue, to follow the purification of an enzyme, or to determine the kinetic parameters of a system. The range of techniques used to measure the rate of an enzyme-catalysed reaction is limited only by the nature of the chemical change and the ingenuity of the investigator. This book describes the design and execution of enzyme assays, covering both general principles and specific chapters. Building upon the highly popular first edition, this book combines revised or rewritten chapters with entirely new contributions. Topics include include experimental protocols covering photometric, radiometric, HPLC, and electrochemical assays, along with methods for determining enzyme assays after gel electrophoresis. The theory underlying each method is outlined, together with a description of the instrumentation, sensitivity and sources of error. Also included are chapters on the principles of enzyme assay and kinetic studies; techniques for enzyme extraction; high- throughout screening; statistical analysis of enzyme kinetic data; and the determination of active site concentration. This second edition of Enzyme Assays will be valuable not only to biochemists, but to researchers in all areas of the life sciences.
Product Details
Amazon Sales Rank: #671394 in Books
Published on: 2002-06-20
Original language: English
Number of items: 1
Binding: Paperback
302 pages
Editorial Reviews
Review Provides a very detailed and useful discussion of practical issues in kinetic analysis, illustrates with relevant examples, and provides primary references to the biochemical literature, which are up-to-date ... It is clearly written and illustrated, and will appeal to professional biochemists interested in enzymes. Natural Product Reports
Review 'Although the approach is practical, underlying theory is explained for each technique and the book will be useful to both laboratory researchers and advanced students.' Aslib Book Guide, Vol. 57, No. 8, August 1992
About the Author Robert Eisenthal is in the Department of Biology and Biochemistry, University of Bath, UK. Michael Danson is in the Department of Biology and Biochemistry, University of Bath, UK.
Customer Reviews
Enzyme Assays Explained
In an age of computerized and automated asay systems, it is possible to gather data without understanding the chemistry or biochemistry involved. This may be suitable for lab techs who work for commercial companies, but it is not a good idea for serious scientists. This book explains how assays work, what can go wrong and what the limitations are. It also reviews the types of instrumentation commonly used in assays. It is an excellent resource for laboratory instructors and graduate students.
A good guide to the principles of enzyme assay development. Enzyme Assays: A Practical Approach is a good guide to the principles of assay development. It contains excellent chapters on the theoretical development of kinetic expressions and the statistical treatment of results. Different assays are illustrated (photometric, radiometric, chromatographic, and electochemical) in their respective chapters that include important considerations for assay development/use with each system. A chapter on buffers and protein determination is also included. Although the book provides some examples (in useful detail) of each kind of assay it is NOT a comprehensive overview. The chapters on electrochemical assays and the techniques for enzyme extraction are incomplete due only to the age of the volume (>5 years old). This book would be an excellent volume for those interested in enzyme assay development and would be suitable for a first year graduate chemistry/biochemistry course.
Enzyme Assays: A Practical Approach is a good guide to the principles of assay development. It contains excellent chapters on the theoretical development of kinetic expressions and the statistical treatment of results. Different assays are illustrated (photometric, radiometric, chromatographic, and electochemical) in their respective chapters that include important considerations for assay development/use with each system. A chapter on buffers and protein determination is also included. Although the book provides some examples (in useful detail) of each kind of assay it is NOT a comprehensive overview. The chapters on electrochemical assays and the techniques for enzyme extraction are incomplete due only to the age of the volume (>5 years old). This book would be an excellent volume for those interested in enzyme assay development and would be suitable for a first year graduate chemistry/biochemistry course.
